Bio-Techne - RnD Systems, GLYCOSYLTRANSFERASE ACTIVITY KIT (1 KIT), EA001

The Glycosyltransferase Activity Kit is a manufacturer-supplied, non-radioactive assay kit for measurement of glycosyltransferase enzyme activity. Quantity: 1 kit. Catalog #: EA001. The kit provides a high-throughput compatible format that uses the nucleotidase ENTPD3/CD39L3 as a coupling phosphatase to remove inorganic phosphate quantitatively from leaving nucleotide diphosphates (e.g., UDP or GDP) generated during glycosyltransferase reactions, enabling accurate kinetic analysis without time-consuming separation steps.

Key Features:

• Quantity: 1 kit
• Catalog number: EA001
• Applicable for glycosyltransferases that use diphosphonucleotide sugars as donor substrates (generate removable phosphate leaving groups)
• Kit contents: Recombinant human ENTPD3/CD39L3 (NS0-expressed), UDP, Phosphatase Buffer 1, 100 mM MnCl2, Phosphate Standard, Malachite Green Reagents A & B
• Assay format: non-radioactive, high-throughput compatible
• Storage: store unopened product at -20 to -70 °C
• Species: Multi-Species

Advantages:

• Enables measurement of a broad range of glycosyltransferases that produce nucleotide diphosphate leaving groups
• Non-radioactive assay chemistry eliminates need for radioactive substrates
• No chromatographic or time-consuming separation steps required, simplifying workflows
• Designed for accurate kinetic analysis and amenable to high-throughput screening formats