Bio-Techne - RnD Systems, ENDOTHELIAL CELL GROWTH MEDIA WITHOUT VEGF (250 ML), CCM029

Endothelial Cell Growth Media without VEGF is a ready-to-prepare kit formulated to support the low-serum, VEGF-free expansion of primary endothelial cells, including human umbilical vein endothelial cells (HUVECs). The product provides an Endothelial Cell Growth Base Media and a 50X Endothelial Cell Growth Supplement without VEGF for preparation of complete media suitable for maintenance and expansion of CD31+, KDR+, and vWF+ endothelial cells in vitro. The kit is supplied as a 250 mL base media format (Catalog CCM029) and is intended for laboratory research use only.

Key Features:

• Volume: 250 mL Endothelial Cell Growth Base Media (kit includes 50X supplement)
• Formulation: VEGF-free; low-serum growth media
• Supplement composition (50X): human FGF basic, human LR3 IGF-1, human EGF, heparin, hydrocortisone, L-ascorbic acid, fetal bovine serum
• Preparation: add 5 mL of 50X supplement to 250 mL base media to make complete media
• Species tested: Human (supports HUVEC growth; CD31+, KDR+, vWF+)
• Storage: unopened product 2–8 °C; completed media stored 2–8 °C (use within 2 weeks)

Advantages:

• Optimized formulation that promotes endothelial cell growth under low-serum, VEGF-free conditions
• Free of VEGF to enable experiments where VEGF exclusion is required
• Includes a single, concentrated supplement for simplified preparation
• 250 mL base media format reduces media waste and supports routine culture workflows