Bio-Techne - RnD Systems, ENDOTHELIAL CELL GROWTH MEDIA (250 ML), CCM027

Endothelial Cell Growth Media is a ready-to-use kit formulated to support the low-serum expansion and maintenance of primary endothelial cells, including human umbilical vein endothelial cells (HUVECs). Supplied as a 250 mL Endothelial Cell Growth Base Media plus a 50X Endothelial Cell Growth Supplement (catalog CCM027), the product provides the nutrients and defined growth factors required for controlled in vitro endothelial cell culture and propagation for research applications.

Key Features:

• Kit includes Endothelial Cell Growth Base Media (250 mL) and Endothelial Cell Growth Supplement (50X)
• Formulated to support human primary endothelial cells (tested with HUVECs; CD31+, KDR+, vWF+)
• Contains optimized concentrations of FGF basic, LR3 IGF-1, VEGF165, EGF, heparin, hydrocortisone, L-ascorbic acid and fetal bovine serum
• Storage: store unopened product at 2–8 °C; supplement shipped/stored at recommended temperature
• Preparation: add 5 mL of 50X supplement to 250 mL base media to prepare complete media

Advantages:

• Optimized, pre‑formulated composition promotes endothelial cell proliferation under low‑serum conditions
• Single 50X supplement simplifies media preparation and reduces hands‑on variability
• Formulation reduces cell senescence at higher passages according to manufacturer testing
• 250 mL base volume minimizes wasted media while supporting multiple culture passages and experiments