Bio-Techne - RnD Systems, CELLXVIVO HUMAN MONOCYTE-DERIVED DC DIFFERENTIATION KIT (1 KIT), CDK004

CellXVivo Human Monocyte-derived DC Differentiation Kit (1 kit, CDK004) is a complete reagent set for the differentiation of human CD14+ monocytes into immature and mature dendritic cells for ex vivo research. The kit provides serum-free dendritic cell base media plus recombinant human IL-4, GM-CSF and TNF-α and includes reagents and buffers required to generate immature dendritic cells in 7 days and to induce maturation with TNF-α for downstream immunological assays. It is intended for generating monocyte-derived dendritic cells for phenotypic analysis and functional assays.

Key Features:

• Quantity: 1 kit (Catalog/Part: CDK004)
• Species: Human
• Reagents supplied: Serum-Free Dendritic Cell Base Media, Recombinant Human IL-4, Recombinant Human GM-CSF, Recombinant Human TNF-α, Reconstitution Buffer 2
• Typical differentiation protocol: 1 x 10^6 CD14+ cells/mL cultured for 7 days; maturation induced with TNF-α for 3 days
• Storage: unopened kit at -20 to -70 °C; opened/reconstituted components 2–8 °C (short term) or -20 to -70 °C (long term)

Advantages:

• Provides optimized, validated reagents to reproducibly generate immature and mature monocyte-derived dendritic cells
• Straightforward procedures that do not require specialized instrumentation
• Includes serum-free base media and key recombinant cytokines for controlled differentiation and maturation