Bio-Techne - RnD Systems, CELLXVIVO HUMAN M2 MACROPHAGE DIFFERENTIATION KIT (1 KIT), CDK013

CellXVivo Human M2 Macrophage Differentiation Kit is a complete reagent kit for differentiating human CD14+ monocytes into M2 macrophages for ex vivo research applications. The kit supplies optimized serum-free base media, recombinant human M-CSF and reconstitution buffer to support a 6-day differentiation protocol and downstream verification by flow cytometry or ELISA. Typical use cases include generation of M2-polarized macrophages for cytokine analysis, phenotypic characterization, and functional studies of anti-inflammatory or tissue-remodeling responses.

Key Features:

• Differentiation target: human CD14+ monocytes into M2 macrophages
• Kit contents: Serum-Free Base Media; Recombinant Human M-CSF; Reconstitution Buffer 2
• Yield: sufficient reagents for two 24‑well plates or 5 × 10^7 cells
• Protocol: suspend cells at 2 × 10^6 cells/mL, culture 6 days, refresh media on day 3
• Storage: store unopened kit at -20 to -70 °C; opened/reconstituted reagents 2–8 °C (short term) or -20 to -70 °C (long term)

Advantages:

• Provides optimized reagents designed to induce consistent M2 macrophage differentiation from human CD14+ monocytes
• Generates M2 macrophages with a CD14+ CD80- CD163+ CD206+ phenotype for reliable phenotypic characterization
• Validated, straightforward procedures that do not require specialized instrumentation, enabling routine laboratory implementation