Bio-Techne - RnD Systems, CELLXVIVO HUMAN B CELL EXPANSION KIT (1 KIT), CDK005

CellXVivo Human B Cell Expansion Kit is a reagent kit for the proliferative expansion of human B cell populations ex vivo. The kit provides optimized reagents and a validated protocol to expand isolated human B cells in culture (culture at 2 x 10^5 cells/mL for 5 days) and is intended for researchers who require increased B cell numbers for downstream assays and ex vivo studies.

Key Features:

• Provides reagents sufficient to expand a starting population of 1 x 10^7 human B cells to a 3–5 fold expanded population
• Reagents supplied: B Cell Expander 1, B Cell Expander 2, B Cell Expander 3, Reconstitution Buffer 1, Reconstitution Buffer 2
• Recommended culture density: 2 x 10^5 cells/mL; incubation period: 5 days
• Species: Human
• Storage (unopened): 2–8 °C; opened/reconstituted expanders: 2–8 °C up to 30 days or -20 to -70 °C up to 3 months; reconstitution buffers stored 2–8 °C up to 12 months

Advantages:

• Yields a 3–5 fold increase in B cell numbers, enabling sufficient cells for downstream applications
• Includes optimized reagents and straightforward, validated procedures to induce B cell proliferation
• Does not require specialized instrumentation for implementation
• Stable storage recommendations provided for unopened and opened/reconstituted components to support reproducible use