Quantabio AccuStart II PCR ToughMix, 100R (SAMPLE) 95142-100

AccuStart II PCR ToughMix is a ready-to-use 2X concentrated PCR SuperMix for amplification of DNA templates, formulated to overcome common PCR inhibitors found in crude environmental, plant, or animal extracts. The product provides stabilized reagents for convenient room-temperature reaction assembly and contains AccuStart II hot start Taq DNA polymerase, enabling sensitive and specific target amplification in standard 25 µL reactions. The 100R (SAMPLE) configuration contains material sufficient for 100 × 25 µL reactions and does not include the GelTrack Loading Dye in the sample size.

Key Features:

• Format: 2X concentrated ready-to-use PCR SuperMix for 25 µL reactions
• Contains: optimized buffer with MgCl2, dNTPs, AccuStart II hot start Taq DNA polymerase, ToughMix additives and stabilizers
• Pack size: 100 reactions (100 × 25 µL reactions, 1 × 1.25 mL)
• Hot-start mechanism: monoclonal antibody-mediated hot start
• Storage: store at -25°C to -15°C; performance unaffected by repeated freeze–thaw

Advantages:

• Room-temperature setup enabled by stabilized 2X SuperMix for faster, convenient reaction assembly
• Stringent antibody hot-start improves specificity and sensitivity of target amplification
• Formulation tolerates many PCR inhibitors commonly present in crude samples, supporting robust amplification
• Stabilized mix resists performance loss from repeated freeze–thaw cycles