Quantabio AccuStart Genotyping ToughMix, L-ROX 1250R 95117-012

AccuStart Genotyping ToughMix, L-ROX is a 2X concentrated master mix formulated for probe-based genotyping assays including SNP detection and allelic discrimination. It provides a ready-to-use reaction cocktail containing AccuStart II ultrapure Taq polymerase with stringent hot-start control, optimized buffer chemistry to improve single base-pair discrimination, and a pre-blended passive reference dye (L-ROX) for instrument normalization. The mix is intended for real-time PCR genotyping directly from challenging sample types and for high-precision allelic discrimination assays.

Key Features:

• Supplied as a 2X concentrated master mix
• Contains AccuStart II ultrapure Taq DNA polymerase with antibody hot-start
• Formulated for probe-based genotyping and SNP allelic discrimination
• Includes pre-blended passive reference dye: L-ROX
• Packaged for 1250 × 20 µL reactions (1250R)

Advantages:

• Buffer chemistry optimized to destabilize single base-pair mismatch probes for superior allelic discrimination and improved cluster separation
• ToughMix formulation neutralizes a broad spectrum of PCR inhibitors enabling reliable performance from crude extracts and complex sample matrices
• AccuStart II enzyme technology delivers sensitive, precise detection with a stringent hot-start to reduce non-specific amplification
• Ready-to-use 2X format with AccuVue loading dye and L-ROX simplifies reaction setup and instrument normalization