Quantabio qScript Ultra SuperMix, 500R 95217-500

qScript Ultra SuperMix is a ready-to-use, single-tube master mix for first-strand cDNA synthesis designed for two-step RT-PCR and RT-qPCR workflows. The SuperMix contains all components required for cDNA synthesis except the RNA template and is formulated to deliver rapid, high-yield, and unbiased representation of RNA sequences, making it suitable for gene expression analysis and RNA pathogen quantification studies. This listing corresponds to the 500-reaction size.

Key Features:

• Format: single-tube qScript Ultra SuperMix for first-strand cDNA synthesis
• Packaging: 500 reactions (500R)
• Part number: 95217-500
• Thermostability: engineered for reverse transcriptase activity up to 65°C
• Reaction time: 10 minute synthesis with a total protocol time of ~13 minutes
• Dynamic range: linear cDNA synthesis from total RNA inputs of 2.5 μg down to 1 pg

Advantages:

• High thermostability enables efficient reverse transcription through challenging RNA secondary structures
• Fast protocol delivers cDNA rapidly (10-minute reaction), increasing throughput
• Wide dynamic range provides sensitive performance across large differences in input RNA
• Single-tube, pre-formulated mix simplifies setup and reduces pipetting steps and handling errors
• Formulation resists common inhibitors (e.g., salts, heparin, melanin) for robust performance with difficult samples