Bio-Techne - RnD Systems, MycoProbe Mycoplasma Detection Kit (1 Kit), CUL001B

The MycoProbe Mycoplasma Detection Kit is a microplate-based colorimetric assay for routine screening of eukaryotic cell cultures for mycoplasma contamination. The kit detects Mycoplasma 16S rRNA and is capable of identifying the eight mycoplasma species known to cause 95% of cell culture contamination. It is intended for high-throughput screening of cell culture supernatants or cell pellets (fresh or frozen) and produces results in approximately 4.5 hours.

Key Features:

• Reagents sufficient for 96 tests (one 96-well plate)
• Detects the eight mycoplasma species that account for ~95% of eukaryotic cell culture contamination
• Sensitivity comparable to PCR (colorimetric signal amplification of 16S rRNA)
• Results in ~4.5 hours
• Compatible with cell culture supernatant or cultured cell pellet samples (fresh or frozen)
• Includes synthetic DNA positive control and pan-specific oligonucleotide probes
• Requires microplate reader capable of measuring absorbance at 490 nm (with 650 or 690 nm correction)

Advantages:

• High-throughput, microplate format enables routine screening of many samples per run
• High sensitivity comparable to PCR while avoiding false positives from amplicon contamination
• Flexible sampling: works with cell pellets or supernatants and with fresh or frozen samples
• Fast turnaround with results in approximately 4.5 hours, supporting timely contamination monitoring