Quantabio SPARQ MRNA-SEQ KIT 95218-024

The sparQ mRNA-Seq Kit is a manufacturer-branded mRNA library preparation kit for Illumina NGS platforms that prepares directional, sequencer-ready mRNA libraries via efficient poly(A) capture. The kit processes 24 reactions per kit and supports rapid, streamlined workflows to convert total RNA into high-quality stranded mRNA-seq libraries from low to moderate input amounts. Typical use cases include transcriptome profiling and gene-expression studies requiring reproducible, directional mRNA libraries for downstream sequencing on Illumina instruments.

Key Features:

• Format: sparQ mRNA-Seq Kit, 24 reactions per kit (95218-024)
• mRNA capture method: efficient poly(A) capture for coding-transcript enrichment
• Input range: generates libraries from low input (as little as 5 ng total RNA) up to 1 µg total RNA
• Library type: directional/strand-specific mRNA libraries compatible with Illumina NGS
• Workflow time: streamlined protocol with approximately 4.75 hours hands-on-to-sequencer-ready library preparation

Advantages:

• High sensitivity for mRNA detection enabling data from low input RNA samples (down to ~5 ng)
• Streamlined, single-day workflow reduces hands-on time and accelerates library preparation
• Produces high-quality, strand-specific libraries that enable reproducible transcript profiling
• Efficient poly(A) capture improves recovery of coding transcripts and reduces non-mRNA background