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Neogen BP0118500 Demi Fraser Broth Base, 500g - 700002158

Neogen -- Item TECPIM000060496
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Neogen Demi Fraser Broth Base is a dehydrated culture media supplied as a 500 g package (Catalog No. BP0118500) formulated for the selective enrichment and rapid detection of Listeria spp. The medium is a modification of UVM/Fraser formulations and is intended as a secondary enrichment broth for isolation of Listeria from food and environmental samples, and for use with ELISA and culture-based workflows. Preparation requires dissolution and sterilization followed by addition of ferric ammonium citrate and selective supplements as specified by the manufacturer.


Key Features:

  • Formulation: dehydrated Demi-Fraser (Half Fraser) broth base for Listeria spp.
  • Mass: 500 g package (Catalog No. BP0118500)
  • Preparation concentration: 57.4 g per liter (yields approximately 9 L from 500 g)
  • Storage conditions: 2 to 30°C
  • Selective components: lithium chloride included in base; ferric ammonium citrate and antibiotic supplements added aseptically after autoclaving

Advantages:

  • Improved selectivity for isolation of Listeria spp. based on the Demi-Fraser/Fraser formulation
  • Compatible with both traditional culture methods and ELISA workflows, with formulation described to give improved results for ELISA
  • Provides visible presumptive detection via esculin hydrolysis producing a black precipitate when ferric ammonium citrate is added

Product Details

Overview
Model BP0118500
Product Type Consumables
Part Number 700002158
Brand Neogen
Gross Dimensions (WxDxH cm)
Net Dimensions (WxDxH cm)
Gross Weight (kg)
Net Weight (kg)
Specifications
Type Enrichment
Packing Pack of 1
Media Type Demi Fraser Broth Base
Document
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